Figure 5 | Scientific Reports

Figure 5

From: Production of kidney organoids arranged around single ureteric bud trees, and containing endogenous blood vessels, solely from embryonic stem cells

Figure 5

Testing mESC-derived components in chimaeric organoids. (a) Schematic of the experiment. (b) Schematic summary of natural expression of markers used in C-E. (c) Chimaeric organoids formed by aggregating whole UB (T shape) with sorted nephron progenitor (NP) and induced stromal cells (iSP) [UB + NP + iSP]. (d) Chimeric organoids formed by aggregating eUB with sorted nephron progenitor (NP) and induced stromal cells (SP) [eUB + NP + SP]. (e) Chimeric organoids formed by aggregating eUB with sorted nephron progenitor (NP) and induced stromal cells (iSP) [eUB + NP + iSP]. Representative bright field (first two images) and immunostaining images are shown. In all cases the ureteric bud (UB/eUB) arborized and all other components developed, but only the UB formed a ureter-type exit tube extending beyond the kidney (c4). Controls are aggregates without either eUB or UB. Scale bar, 200 µm for bright field images and 100 µm for fluorescence images. Abbreviations: CD31: platelet endothelial cell adhesion molecule, CK8: cytokeratin 8, WTI: Wilms tumor 1, JAG: jagged1, PCK: pan-cytokeratin, POD: podocalyxin, URO: uroplakin. Images are representative of at least three different experiments.

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