Figure 1
Beovu lowered the cell index of iBREC. iBREC were cultivated on gold electrodes until confluency was reached before (a), (b) Beovu (n = 7), (a), (c) Lucentis (n = 6), (d) nintedanib (n = 7), (e) tivozanib (n = 3), or phosphate-buffered saline (control, blue curves; n ≥ 6) was added and the cell index recorded continuously as a measure of barrier function. Cell index values were normalized in relation to those measured immediately before addition of the VEGF-A antagonists and are shown as means with standard deviations. Data were analyzed with two-way ANOVA followed by Sidak’s multiple comparison test. (a), (b) Beovu significantly lowered the cell index values transiently early after its addition and persistently from ~ 1.5 days of exposure until the end of the experiment over seven days. (a), (c) Cell index values were not different in the presence of Lucentis. (d) Nintedanib (10 nM) significantly enhanced cell index values late after its addition, whereas the higher concentration (100 nM) had no effect. (e) Cell index values were also not changed by 10 nM tivozanib.
