Table 1 Measured cytokine concentrations in cell culture supernatants.
Target | Source of ELISA kit | Concentration ranges of standard curves (pg/ml) | Minimal detectable dose (pg/ml) | Measured concentration of target after addition of 1 mg/ml Beovu (pg/ml) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
2 h | 30 h | 5 d | 8 d | |||||||||||
Control | Sample | Control | Sample | Control | Sample | Control | Sample | |||||||
VEGF-A | CAVE00, bio-techne | 0 | – | 1250 | 20 | ≤ 0 (N = 6) | ≤ 0 (N = 6) | ≤ 0 (N = 7) | ≤ 0 (N = 7) | ≤ 0 (N = 3) | ≤ 0 (N = 3) | ≤ 0 (N = 8) | ≤ 0 (N = 8) | |
TNFα | EBTNF, Invitrogen*) | 0 | – | 30,000 | 120 | ≤ 0 (N = 8) | ≤ 0 (N = 8) | ≤ 0 (N = 8) | ≤ 0 (N = 8) | ND*) | ND | ≤ 0 (N = 6) | ≤ 0 (N = 6) | |
IL-1β | ESS0027, Thermo Fisher Scientific | 0 | – | 2000 | 31 | ≤ 0 (N = 8) | ≤ 0 (N = 8) | ≤ 0 (N = 8) | ≤ 0 (N = 8) | ND | ND | ND | ND | |
IL-6 | ESS0029, Invitrogen | 0 | – | 5000 | 78 | Below minimal detectable dose | ||||||||
(38 ± 7) (N = 8) | (42 ± 9) (N = 8) | (3 ± 5) (N = 8) | (3 ± 18) (N = 8) | (3 ± 7) (N = 7) | (11 ± 22) (N = 7) | (42 ± 52) (N = 6) | (62 ± 55) (N = 6) | |||||||
IL-8 | ECCXCL8, Invitrogen | 0 | – | 1500 | 38 | ≤ 0 (N = 8) | ≤ 0 (N = 8) | ≤ 0 (N = 8) | ≤ 0 (N = 8) | ND | ND | ≤ 0 (N = 6) | ≤ 0 (N = 6) | |
