Table 1 Experimental design of factorial design parameters obtained for independent variables: X1 (alcalase to flavourzyme ratio, w/w), X2 (time, min) and experimental values of responses: DH (%), protein content (%) and area of molecular weight (mAU*S).
Factors | DH (%) | Protein (%) | Area of molecular weight (mAu*S) | |||
|---|---|---|---|---|---|---|
Ratio of Fl:Al (%) | Hydolysis time (min) | 5–10 kDa | 3–5 kDa | < 3 kDa | ||
0:100.0 | 60 | 12.05 | 39.51 ± 1.33 | 673.62 ± 7.07 | 414.92 ± 4.24 | 279.24 ± 7.28 |
7.5:92.5 | 60 | 7.09 | 42.36 ± 0.63 | 770.12 ± 12.03 | 469.32 ± 1.41 | 367.24 ± 3.54 |
15.0:85.0 | 60 | 7.79 | 42.17 ± 0.24 | 724.27 ± 21.92 | 505.51 ± 5.66 | 355.01 ± 8.49 |
0:100.0 | 300 | 17.74 | 34.40 ± 0.14 | 683.21 ± 2.83 | 419.65 ± 4.24 | 272.42 ± 1.41 |
7.5:92.5 | 300 | 12.90 | 37.22 ± 0.18 | 857.67 ± 23.33 | 482.48 ± 5.66 | 348.53 ± 6.36 |
15.0:85.0 | 300 | 12.79 | 38.76 ± 0.14 | 659.86 ± 16.26 | 416.59 ± 2.12 | 292.44 ± 13.44 |
0:100.0 | 540 | 19.31 | 36.51 ± 0.51 | 751.85 ± 8.49 | 438.20 ± 7.07 | 285.51 ± 1.41 |
7.5:92.5 | 540 | 14.50 | 38.09 ± 0.10 | 752.75 ± 2.83 | 454.44 ± 10.61 | 309.78 ± 7.07 |
15.0:85.0 | 540 | 14.77 | 38.19 ± 0.25 | 741.07 ± 7.07 | 413.75 ± 4.95 | 288.42 ± 7.78 |
7.5:92.5 | 300 | 13.80 | 36.57 ± 0.14 | 834.09 ± 4.61 | 470.83 ± 1.83 | 332.26 ± 12.57 |
7.5:92.5 | 300 | 14.60 | 37.87 ± 0.14 | 857.88 ± 2.81 | 488.65 ± 9.23 | 353.23 ± 5.77 |
7.5:92.5 | 300 | 14.40 | 38.76 ± 0.24 | 815.94 ± 26.64 | 451.62 ± 0.13 | 339.89 ± 16.28 |
7.5:92.5 | 300 | 14.20 | 37.84 ± 0.12 | 835.33 ± 15.58 | 486.88 ± 11.72 | 336.36 ± 1.35 |
