Table 3 Regression coefficient (β) and adjusted coefficient of determination (adj. R2) of the predicted second-order model for the response variables.
Responses | Regression coefficients (β) | ||||||
|---|---|---|---|---|---|---|---|
DH | Protein | 3–5 kDa | < 3 kDa | DPPH | ABTS | FRAP | |
Constant | |||||||
X0 | 9.61 | 41.43 | 405.57 | 287.67 | 5.44 | 1.12 | 793.24 |
Linear | |||||||
X1 | − 0.83 | 0.48 | 15.96 | 18.15 | − 0.04 | 1.78 × 10–3 | 18.05 |
X2 | 2.47 | − 0.03 | 0.06 | − 0.08 | 0.02 | − 8.52 × 10–4 | − 1.30 |
Quadratic | |||||||
\({\mathrm{X}}_{1}^{2}\) | 0.03 | − 0.02 | − 0.65 | − 0.86 | – | – | − 1.15 |
\({\mathrm{X}}_{2}^{2}\) | − 0.16 | 4.41 × 10–5 | – | 1.32 × 10–4 | − 3.51 × 10–5 | 1.74 × 10–4 | 1.47 × 10–3 |
Interaction | |||||||
X12 | – | – | − 0.02 | − 0.01 | – | – | – |
Adj. R2 | 0.9629 | 0.8742 | 0.6870 | 0.8642 | 0.6311 | 0.7585 | 0.8981 |
F-value | 78.76 | 21.84 | 7.58 | 16.28 | 7.84 | 13.56 | 27.45 |
P-value | < 0.0001 | 0.0002 | 0.0079 | 0.0010 | 0.0070 | 0.0011 | 0.0001 |
Lack of fit | 0.5696 | 0.5142 | 0.2716 | 0.1394 | 0.2368 | 0.7565 | 0.5998 |