Table 1 Concentration of the sixteen independent nutrient variables screened by Plackett–Burman.

From: Agro-waste as a substrate for the production of pullulanase by Penicillium viridicatum under solid-state fermentation

SN

Nutrients

− 1 (%)

(g/g dry substrate)

+ 1 (%)

(g/g dry substrate)

1

Peptone

0.1

0.2

2

Yeast extract

0.1

0.2

3

NH4SO4

0.1

0.2

4

Urea

0.1

0.2

5

KH2PO4

0.1

0.2

6

Na2CO3

0.1

0.2

7

CuSO4

0.05

0.1

8

FeSO4

0.05

0.1

9

CaCl2

0.05

0.1

10

MnSO4

0.05

0.1

11

MgSO4

0.05

0.1

12

KCl

0.05

0.1

13

ZnSO4

0.05

0.1

14

NaCl

0.05

0.1

15

KHSO4

0.1

0.2

16

NaNO3

0.1

0.2

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