Table 3 Nutrient contributions to pullulanase production by Placket-Burman design matrix (randomized).

From: Agro-waste as a substrate for the production of pullulanase by Penicillium viridicatum under solid-state fermentation

Std

Run

A

B

C

D

E

F

G

H

J

K

L

M

N

O

P

Q

R

S

T

Response: Enzyme activity (U/gds)

Factors (coded values)

13

1

1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

− 1

1

1

− 1

− 1

1

1

1

40.00

7

2

− 1

− 1

− 1

1

1

− 1

1

1

− 1

− 1

1

1

1

1

− 1

1

− 1

1

− 1

41.00

2

3

− 1

1

1

− 1

− 1

1

1

1

1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

43.00

11

4

1

− 1

1

− 1

− 1

− 1

− 1

1

1

− 1

1

1

− 1

− 1

1

1

1

1

− 1

41.18

6

5

− 1

− 1

1

1

− 1

1

1

− 1

− 1

1

1

1

1

− 1

1

− 1

1

− 1

− 1

43.73

19

6

1

− 1

− 1

1

1

1

1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

− 1

1

38.00

15

7

1

1

1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

− 1

1

1

− 1

− 1

1

29.57

18

8

− 1

− 1

1

1

1

1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

− 1

1

1

39.45

9

9

1

− 1

− 1

− 1

− 1

1

1

− 1

1

1

− 1

− 1

1

1

1

1

− 1

1

− 1

30.00

17

10

− 1

1

1

1

1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

− 1

1

1

− 1

38.15

10

11

− 1

1

− 1

− 1

− 1

− 1

1

1

− 1

1

1

− 1

− 1

1

1

1

1

− 1

1

51.67

4

12

1

1

− 1

1

1

− 1

− 1

1

1

1

1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

56.25

20

13

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

21.23

8

14

− 1

− 1

− 1

− 1

1

1

− 1

1

1

− 1

− 1

1

1

1

1

− 1

1

− 1

1

38.00

16

15

1

1

1

1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

− 1

1

1

− 1

− 1

35.67

12

16

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

− 1

1

1

− 1

− 1

1

1

1

1

40.35

3

17

1

− 1

1

1

− 1

− 1

1

1

1

1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

47.00

1

18

1

1

− 1

− 1

1

1

1

1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

− 1

47.00

14

19

1

1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

− 1

1

1

− 1

− 1

1

1

37.91

5

20

− 1

1

1

− 1

1

1

− 1

− 1

1

1

1

1

− 1

1

− 1

1

− 1

− 1

− 1

39.62

22

21

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

28.54

21

22

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

25.44

23

23

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

27.33

  1. Symbols used: A-peptone; B-Yeast extract; C-NH4SO4,; D-urea; E-KH2PO4; F-Na2CO3; G-CuSO4; H-FeSO4; J-CaCl2; K-MnSO4; L-MgSO4; M-KCl; N-ZnSO4; O-NaCl; P-KHSO4; Q-NaNO3; R- Dummy 1; S-Dummy 2; T-Dummy.