Table 4 Box Behnken design showing effect of various factors on pullulanase production.

Run	FeSO₄	MnSO₄	MgSO₄	Actual enzyme activity (U/gds)	Predicted enzyme activity (U/gds)
1	0.4	0.4	0.3	50.83	50.93
2	0.3	0.3	0.3	62.3	61.84
3	0.3	0.3	0.3	62.4	61.84
4	0.3	0.2	0.4	57.46	58.06
5	0.3	0.3	0.3	61.00	61.84
6	0.4	0.3	0.4	49.70	49.42
7	0.2	0.3	0.4	58.63	58.12
8	0.3	0.4	0.2	60.32	59.71
9	0.4	0.3	0.2	55.89	56.39
10	0.2	0.4	0.3	58.63	58.95
11	0.2	0.2	0.3	52.64	52.54
12	0.3	0.4	0.4	48.44	48.61
13	0.3	0.3	0.3	61.4	61.84
14	0.3	0.3	0.3	62.1	61.84
15	0.4	0.2	0.3	55.89	55.56
16	0.2	0.3	0.2	52.41	52.68
17	0.3	0.2	0.2	48.67	48.49

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