Figure 1 | Scientific Reports

Figure 1

From: Specific egg yolk antibody raised to biofilm associated protein (Bap) is protective against murine pneumonia caused by Acinetobacter baumannii

Figure 1

The confirmation of recombinant protein (Bap) expression by western blotting analysis (A), and purified IgY antibodies by SDS-PAGE analysis (B, C). (A) The result showed a band with a molecular weight of 45 kDa, Lane 1: Protein marker, Lane 2: Bap. (B) The confirmation of purified IgY antibodies by SDS-PAGE analysis under the non-reducing condition, 10% SDS-PAGE gel showed a band (180 kDa), Lane 1: IgY-Bap, Lane 2: IgY- control, Lane 3: Protein marker. (C) The confirmation of purified IgY antibodies by SDS-PAGE analysis under the reducing condition, two bands on 10% SDS-PAGE gel are the heavy chain (75 kDa) and the light chain (25 kDa) of IgY, Lane 1: Protein marker, Lane 2: IgY-Bap, Lane 3: IgY- Control.

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