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Figure 1

From: Whole structural reconstruction and quantification of epidermal innervation through the suction blister method and skin-clearing technique

Figure 1

Summarized processes of advanced skin-clearing protocol. The epidermis was separated from the dermis using the (a) suction blister method instead of (b) a previously adopted SSST8. The separated epidermis subsequently proceeded to the cutaneous ACT-PRESTO procedures, including (c) fixation, (d) electrophoretic tissue clearing (ETC), and (e) RI matching. SSST Salt-split skin test, ACT Active clarity technique, PRESTO Pressure-related efficient and stable transfer of macromolecules into organs, RI Reflective index, RIMS Reflective index matching solution.

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