Figure 1 | Scientific Reports

Figure 1

From: DJ-1 interacts with the ectopic ATP-synthase in endothelial cells during acute ischemia and reperfusion

Figure 1

Endothelial DJ-1 content declines during I/R injury. ECs cultures were subjected to either 30 min or 1 h in vitro ischemia followed by 2 or 22 h of reperfusion, and both protein and RNA extracts were probed for DJ-1. (A) Representative western blot. (B) Quantification of endothelial full length DJ-1 upon I/R. (n = 5; **p < 0.01). (C) Quantification of endothelial DJ-1∆C upon I/R (n = 5).; and, (D) park7 gene expression upon I/R in ECs (n = 3). Data presented as mean ± SEM. ECs endothelial cells, I/R ischemia–reperfusion. Corresponding uncropped western blot acquisitions can be found in Supplemental Fig. 2.

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