Figure 3
Extracellular ATP generation after ischemia is dependent on DJ-1. ECs cultures were transfected with a siRNA targeted to DJ-1, and subjected to 1 h in vitro ischemia. Extracellular ATP generation was then evaluated. (A) Representative western blot showing DJ-1 inhibition 72 h post-transfection. (B) Western blot quantification of DJ-1 72 h post-transfection (n = 4; *p < 0.05). (C) park7 gene expression of control and knock-down cultures 72 h post-transfection (n = 3; **p < 0.01). (D) Extracellular ATP generation of DJ-1 knock-down and control cultures after 1 h in vitro ischemia compared to normoxic controls (n = 4; *p < 0.05; n.s. not significant). Data presented as mean ± SEM. ECs endothelial cells. Corresponding uncropped western blot acquisition can be found in Supplemental Fig. 4.
