Figure 5
ATP-synthase co-immunoprecipitation with exogenous DJ-1 and DJ-1∆C in ischemia and I/R. ECs cultures were exposed to either 1 h ischemia or normoxia, in the presence and the absence of exogenously administrated DJ-1 or DJ-1∆C at 100 nM, with and without a reperfusion period of 2 h and 24 h (whithout exogenous DJ-1/DJ-1∆C). After indicated treatment, cells were thoroughly rinsed, lysed, and immunoprecipitated against ATP-synthase. (A) Western blot analysis of ATP synthase (ATP5B) and DJ-1/DJ-1∆C in whole cell lysates (immunoprecipitation input). (B) Western blot analysis of ATP synthase (ATP5B) and DJ-1/DJ-1∆C in the IP-captures. ECs endothelial cells, IP immunoprecipitate, I/R ischemia/reperfusion. Membranes were split in two before incubation. Corresponding uncropped western blot acquisitions can be found in Supplemental Fig. 5.
