Figure 3
Effect of leptin and palmitic acid on Fetuin B expression in hepatocytes. The Fetuin B protein and mRNA levels were increased by treatment with leptin for 16 h in primary murine hepatocytes (a, b). The Fetuin B protein and mRNA levels were increased by treatment with leptin for 12 h in AML12 cells (c, d). Fetuin B protein and mRNA levels increased upon palmitic acid stimulation for 16 h in primary murine hepatocytes (e, f). The Fetuin B protein and mRNA levels were increased by palmitic acid stimulation for 12 h in AML12 cells. (g, h). The mRNA level of LepRb was analysed by qRT–PCR after palmitic acid stimulation in primary murine hepatocytes for 16 h (i) and AML12 cells for 12 h (j). qPCR data are presented as the mean ± SEM of two or three independent experiments. Western blot images are representative of two or more independent experiments. Quantitation of the representative western blot is presented as the mean ± SEM of triplicate measurements of the grey intensity ratio of Fetuin B/loading control. Original blots for independent replications for each experiment (n = 2–4) are presented in the Supplementary Information file. One-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test was used for experiments with three or more groups. t test was used to compare two groups. *p < 0.05 compared with the control group; **p < 0.01 compared with the control group; ***p < 0.001 compared with the control group, ****p < 0.0001 compared with the control group.
