Figure 1

Myofiber distribution and CSA relative to ROM. Representative merged photomicrographs of co-labeled T1 (green), and T2 (red) myofibers in AG sections from (A) good, (B) fair, and (C) poor ROM patient groups were segmented by individual (A’–C’) T1, and (A”–C”) T2 grayscale channels for fiber count and CSA analyses using Slidebook 5 and Graphpad PRISM 9 software for morphometry and statistics, respectively. Bars = 50 µm. (D) Mean ± SD fiber counts between and within ROM groups. (E) Spearman’s ρ (r) was calculated between T2 (and correspondingly T1) fibers and total ROM. Data points were shaded according to severity of FxC, and r calculated between FxC and T2 content. (F) Mean CSAs were compared within and between ROM groups and (G) tested for associations with both total ROM and FxC. *p < 0.05 between groups and #p < 0.05 within groups; **p < 0.01; ***p < 0.001; and ****p < 0.0001.