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Figure 1

From: c-di-AMP signaling plays important role in determining antibiotic tolerance phenotypes of Mycobacterium smegmatis

Figure 1

Modulating intracellular c-di-AMP concentration affects the number of spontaneous resistant mutants against ciprofloxacin and rifampicin. (A) ciprofloxacin mutation frequency and (B) rifampicin mutation frequency were calculated for M. smegmatis WT, M. smegmatis ΔdisA, M. smegmatis Δpde and their respective complementation strains [N = 3]. All the strains were grown till stationary phase. Similarly, (C) ciprofloxacin mutation rate and (D) rifampicin mutation rate were calculated for M. smegmatis WT, M. smegmatis ΔdisA, M. smegmatis Δpde along with respective complementation strains [N = 3]. All the graphs are plotted using GraphPad Prism8, unpaired t-test was used to calculate statistical significance: ***P < 0.001; **P < 0.01; *P < 0.05; ns = non- significant.

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