Table 2 Results of MANOVA and post-hoc analyses on the effect of the factors pyrolysis temperature of DOR-based biochar (350 and 500 °C), application dose of DOR-based biochar (2 and 5%), soil contamination level (“low”, “medium” and “high”), soil treatment time (30, 60 and 90 days), inoculation of F. mosseae (AMF) and their interactions on soil enzyme activities (β-glucosidase and dehydrogenase) considering only amended microcosms.
Factors | β-glucosidasea | Dehydrogenaseb |
|---|---|---|
DOR pyrolysis temperature (P) | ||
Fsignificance (df)c | 10.81*** (1) | 7.52*** (1) |
Post-hoc test | ||
350 °C | 1162.88 a | 0.40 b |
500 °C | 1200.48 b | 0.39 a |
Biochar application dose (D) | ||
Fsignificance (df)c | 51.80*** (1) | 15.86*** (1) |
Post-hoc test | ||
2% | 1232.74 b | 0.38 a |
5% | 1148.28 a | 0.41 b |
Soil contamination level (S) | ||
Fsignificance (df)f | 46.46*** (2) | 5044.91*** (2) |
Post-hoc test | ||
Low | 1436.14 b | 0.45 b |
Medium | 1122.88 a | 0.46 b |
High | 1229.23 a | 0.14 a |
Soil treatment time (T) | ||
Fsignificance (df)f | 936.05*** (2) | 22.84*** (2) |
Post-hoc test | ||
30 days | 1100.52 a | 0.41 b |
60 days | 1191.51 b | 0.42 b |
90 days | 1317.82 c | 0.38 a |
F. mosseae inoculation (M) | ||
Fsignificance (df)f | 0.51 (1) | 18.17*** (1) |
Post-hoc test | ||
No inoculation | 1196.07 a | 0.44 b |
Inoculation | 1183.59 a | 0.38 a |
Significant interactions | P × D*, P × M*, D × M*, S × T***, T × M***, P × D × S***, P × S × T*, P × D × M*, D × S × M*** | D × S*, D × T***, D × M*, S × T***, S × M***, M × T*, P × D × T***, D × S × T***, D × S × M***, D × T × M***, S × T × M***, P × D × S × T***, D × S × T × M*** |
