Figure 6

In vivo expression of cluster markers Clusterin and Gpr17 in OPCs. (A) RNAscope expression of Pdgfrα (red), Olig1 (green), Clu (white), and Hoechst (blue). White dotted line indicates the nuclear outline. (B) Quantification of Clu+ and Clu− OPCs (depicted in (A); N = 2 independent experiments, n = 4 individual mice (2 males and 2 females) with 206 total cells analyzed). Each sample includes quantification of OPCs from the cortex, hippocampus, corpus callosum, and cerebellum. (C) RNAscope expression of Pdgfrα (red), Olig2 (green), Gpr17 (white), and Hoechst (blue). White dotted line indicates the nuclear outline. (D) Quantification of Gpr17+ and Gpr17− OPCs (depicted in (C); N = 2 independent experiments, n = 3 individual mice (1 male and 2 females) with 204 total cells analyzed). Each sample includes quantification of OPCs from the cortex, hippocampus, corpus callosum, and cerebellum. In all RNAscope experiments, OPCs were identified as cells co-expressing an OPC surface marker (Pdgfrα or Cspg4) and an oligolineage transcription factor (Olig1 or Olig2). Scale bar = 10 µm.