Figure 2
From: Molecular characterization of polyphenol oxidase between small and large leaf tea cultivars
Leaf appearance, CsPPO transcript level, and CsPPO enzyme activity in the young and old leaves of small- and large-leaf varieties. Photo of (a) young and (b) old leaves of small- and large-leaf varieties. CsPPO transcript levels of the (b) young and (e) old leaf tissues of small- and large-leaf varieties. PPO enzyme activity in (c) young and (f) old leaf tissues of small- and large-leaf varieties. Total RNA was extracted from the young and old leaves of each variety. CsPPO transcript levels were measured using qRT-PCR and normalized to that of 18S rRNA. One unit of PPO activity was defined as the amount of enzyme required to metabolize pyrocatechol into a micromole of benzoquinone per minute. Values are presented as the mean ± SD of three biologically independent experiments. Different letters indicate significant difference between varieties assessed using the LSD post hoc test (p < 0.05). Bar = 5 cm.
