Figure 6
Differential hepatic DNA-methylation in Mup knockout (KO) mice and integration with transcriptomic data. (a) Average CpG methylation level distribution of male (m) and female (f) KO mice (grey) and their wildtype (WT) counterparts (black). (b) Heatmap showing the significant (p < 0.05) hypo- and hypermethylated differentially methylated regions (DMR) with > 5% methylation difference in male mice. Color gradient corresponds to individual DMR methylation status (red: hypermethylated, blue: hypomethylated) per sample and corresponding gene expression level retrieved from KO vs. WT differential gene expression analysis (green: upregulated, grey: downregulated). (c) Venn diagram showing no overlap between DMR and differentially expressed genes (DEG; p < 0.01). (d) Residual of predicted and biological age among mKO and mWT mice. Data (a) and (d) are presented as Spear style boxplots, where the whiskers indicate the minimum and maximum value, lower and upper hinges correspond to the upper and lower quartiles, and the center line to the median. (a) mWT = 6, mKO = 5, fWT = 6, fKO = 6; (b–d) mWT = 6, mKO = 5.
