Figure 4
KMS-12-BM-PPR are more sensitive to BCL-2 inhibition than the parental cells. (a) Lactate production was measured over time and normalized for cell count. (b) Oxygen consumption rate was measured before and after exposure to the inhibitors of the electron transport chain and oxidative phosphorylation. (c) Cells were treated continuously for 48 h with ABT-737 before being assayed for viability with Alamar Blue (n = 2). (c) BPR cells are more sensitive to a Bcl-2-specific inhibitor ABT-199, but not Bcl-XL specific inhibitor. Left, cells were treated for 48 h with ABT-199 (left) or Bcl-XL inhibitor (A-1155463) before viability was determined by Alamar Blue (n = 2–3). (d) Cells were treated with Btz for 1 h and then with ABT-199 for 47 h before viability was determined by Alamar Blue (n = 2–4). The numbers on the graph are combination indexes (CI). (e) Expression of Bcl-2 family member proteins was determined by westerns following 1 h pulse treatment with Btz and overnight recovery. Uncropped images are presented on Fig. S3c. (f) Cells were treated for 48 h with Mcl-1 inhibitor S63845 before viability was determined by Alamar Blue (n = 2).
