Figure 2

Emergence of hematopoietic and erythroid progenitors in gastruloids. (a) Representative flow cytometry plots and (b) relative quantification of the emergence of the c-Kit⁺/CD34⁺ populations in gastruloids from 96 to 168 h. The c-Kit⁺/CD34⁺ population was gated further for TER119⁻/CD41⁺ populations, with (c) representative flow cytometry plots and (d) relative quantification. We refer to the remaining cells after gating for c-Kit⁺/CD34⁺ cells as Not(c-Kit⁺/CD34⁺). Notably, the Not(c-Kit⁺/CD34⁺) population still contains single positive cells for c-Kit and CD34. The Not(c-Kit⁺/CD34⁺) population was additionally gated for TER119⁺/CD41⁻, with (e) representative flow cytometry plots and (f) relative quantification. The graphs in (b, d, f) show the changes in the percentage of positive cells over time. Flk1-GFP (Flk1) (n = 2 replicates), Gata6-Venus (Gata6) (n = 2 replicates) and Sox1-GFP::Brachyury-mCherry (SBr) (n = 1 replicate). Data are represented as mean ± SD.