Figure 3

Emergence of blood progenitors in gastruloids. (a) Representative light-sheet images of Gata6-Venus gastruloids at 168 h showing the co-expression of CD31 and Sox17 in a 3D reconstruction and (b) in a single Z-plane. (c) Representative light-sheet images of Gata6-Venus gastruloids at 168 h showing the co-expression of CD34 and Sox17 in a 3D reconstruction and (d) a single Z-plane. (e) Representative light-sheet images of Gata6-Venus gastruloids at 168 h showing CD41⁺ clusters that arise near the CD31⁺ vascular network. (f) Representative sections of Gata6-Venus gastruloids at 168 h show CD41⁺ cells at higher magnification. Scale bars,100 µm.