Figure 3
Induction of naive iPSCs state, EB differentiation and gene expression analysis. Timeline of in vitro conversion of primed-hiPSCs into naive-hiPSCs and PGC-LCs differentiation (a). RT-qPCR analysis for detection of the pluripotency marker NANOG (b); early PGC marker genes SOX17 and NANOS3 (c). Relative expression levels are shown with normalization to RPLPO gene. Histograms represent mean from independent biological replicates represented by each point. Mann–Whitney tests were used for PCR analysis. Mann–Whitney tests were performed to compare expression values. *p < 0.05. BMP2/4 bone morphogenetic protein 2/4, EB embryoid body, EB D4 embryoid body day 4, EGF epidermal growth factor, GSK3 glycogen synthase kinase-3, hiPSCs human induced pluripotent stem cells, iPSCs induced pluripotent stem cells, JNK c-Jun N-terminal kinase, LIF leukemia inhibitory factor, MAPK mitogen-activated protein kinases, MEK mitogen-activated protein kinase kinase, SCF stem cell factor, TGF-β1 transforming growth factor β1.
