Figure 1 | Scientific Reports

Figure 1

From: Validation and advantages of using novel RT-qPCR melting curve analysis assays for the identification of SARS-CoV-2 variants

Figure 1

Created with BioRender.com The binding of the wild type and mutation sequence to the mutation specific probe. The probe has higher affinity for the mutation sequence and will result in a higher melting point temperature (Tm). The probe has a lowered affinity for the WT sequence due to the single nucleotide mismatch resulting in a decreased Tm.

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