Figure 7 | Scientific Reports

Figure 7

From: Validation and advantages of using novel RT-qPCR melting curve analysis assays for the identification of SARS-CoV-2 variants

Figure 7

Five samples for the P.1 + P681H, B.1.351, B.1.1.7, B.1.258 and WT were analyzed. The melting curve of the five samples are illustrated for the K417N/T assay. As it is shown in the graph the K417N mutation can be distinguished by approximately 4 °C from the WT sequence and the K417T mutation can be distinguished 4 °C below the WT sequence.

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