Table 3 Melting temperatures boundaries for mutations and WT sequences for the five assays.

From: Validation and advantages of using novel RT-qPCR melting curve analysis assays for the identification of SARS-CoV-2 variants

N501Y

P681H

E484K

WT

Mutation

WT

Mutation

WT

Mutation

56.0–60.5 °C

 > 62.0 °C

55.0–58.5 °C

 > 64.0 °C

52.0–55.0 °C

 > 60.0 °C

N439K

K417N/T

WT

Mutation

WT

Mutation N

Mutation T

52.5–55.5 °C

 > 58.0 °C

59.0–61.5 °C

 > 63.5 °C

55.0–56.5 °C

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