Figure 2

TSO IIA treatment decreased the clustering of AQP4 in the plasma membrane after OGD/R. (A) Western blot analysis showing the AQP4 protein in the plasma membrane after OGD/R in untreated cells or cells treated with TSO IIA. (The data are expressed as mean ± SEM, n = 5 independent cultures. One-way ANOVA resulted in P < 0.01, **P < 0.01 versus control; ##P < 0.01 versus OGD/R). (B) Double-labeling immunofluorescence staining confirmed AQP4 protein clustering in the plasma membrane under different treatment conditions (n = 5 independent cultures).