Table 1 Sequences of design primers and hydrolysis probes.

From: Validation of droplet digital PCR for cytokeratin 19 mRNA detection in canine peripheral blood and mammary gland

Gene

Accession number

5′-sequence-3′

Product size in base pairs

Genomic location (CanFam3.1 GCF_000002285.3)

CK19

NM_001253742.1

Forward CCAAGATTGTCCTGCAGATTGAC

Reverse GCCTGCTCCGTCTCAAACTT

Probe FAM-CCCGTCTGGCTGCGGATGACTTC-BHQ1

76

NC_006591.3 (21240778..21245039)

HPRT

AY283372.1

Forward CGAGATGTGATGAAGGAAATGG

Reverse AGCAGGTCAGCAAAGAATTTATAGC

Probe Hex-CCATCACATCGTAGCCCTCTGTGTGC-BHQ1

86

NC_006621.3 (105115732..105153702)

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