Figure 3
From: A maternal high-fat diet induces fetal origins of NASH-HCC in mice
HORMAD1 colocalizes with HIF-1α in fetal livers. (A) Representative photomicrographs of immunohistochemical staining for HIF-1α and HORMAD1 in fetal livers at 14.5 dpc. Scale bars: 300 μm (low magnification) and 30 μm (high magnification). Insets show the magnified images from the left panels. (B) Western blot analysis showed positive correlations between HORMAD1 and HIF-1α levels in the fetal livers of mice (R2 = 0.8469, p = 0.0012). (C) Immunohistochemical staining for HIF-1α and HORMAD1 in the livers of offspring at 15 weeks of age. Scale bars: 300 μm (low magnification) and 30 μm (high magnification). Quantification of HORMAD1-positive area (CD = 8 offspring from 3 dams, HFD = 7 offspring from 3 dams). **p < 0.01 using the Mann–Whitney test. (D) Western blot analysis of HIF-1α and HORMAD1 levels in the livers of 15-week-old offspring. MWM: 117 kDa band in the molecular weight marker (HiMark Prestained protein standard, Thermo Fisher Scientific). (E) Macroscopic image of the liver after barium perfusion of offspring from HFD-fed dams at 33 weeks old. (F) Visualization of 3D vessels in the liver of offspring from HFD-fed dams at 33 weeks old using synchrotron micro-CT and Amira software (version 1.4.0, https://www.thermofisher.com). (G) Immunohistochemical staining for HIF-1α and HORMAD1 in hepatic tumors from 33-week-old offspring of HFD-fed dams. Insets in the middle panels show the magnified images (right panels). HORMAD1 expression in peritumoral lesions (dotted line) and intratumoral lesions (double lines) in hepatic tumors from 33-week-old offspring of HFD-fed dams. Insets in the left panels show the magnified image of each lesion. (H) Capsular invasion of a hepatic tumor. Arrows show the area of invasion. Bars: 300 μm (low magnification) and 30 μm (high magnification).
