Figure 4
From: MicroRNA-101a enhances trabecular bone accrual in male mice
In vivo induction of miR-101a is reversible. Three-week-old R26/101a male mice were fed regular (reg, R) or doxycycline-supplemented diet (dox, D) to assess the reversibility of miR-101a induction in vivo. Schematic representation of the mouse feeding regiment beginning at three and ending at eight weeks of age (A). Expression of miR-101-5p and miR-181a-3p (B) as well as GFP and Akt1 (C) in femora of eight-week-old mice (n = 2 to 4); miR-181a was arbitrarily included as a representative miRNA with a different expression pattern to reflect selectivity in the upregulation of miR-101-5p. MiRNA expression levels are presented using miR-103a-3p as internal control for normalization.
