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Figure 1

From: Isolation and characterization of phage display-derived scFv antibodies against human parechovirus 1 VP0 protein

Figure 1

Time-resolved fluoroimmunoassay of selected scFv clones. Based on CDR-H3 sequences, a total of 11 clones were selected for secondary screening against VP0-GST and GST in a TRF-assay using europium chelate. In the assay, His-tag purified scFv clones expressed as a fusion to bacterial alkaline phosphatase, His-tag and FLAG tag were bound to biotinylated VP0-GST and biotinylated GST on 96 well streptavidin microtiter plate. Bound scFv-AP was detected with polyclonal Eu-labelled anti-alkaline phosphatase antibody and time-resolved fluorescence from europium was measured with Victor3 multilabel counter. The Y-axis represents the europium counts per second. The X-axis represents the different clones screened based on different CDR-H3 sequence.

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