Figure 3

Detection of PeV-A1-infected HT-29 cells with scFv-71. HT-29 cells were mock-inoculated or inoculated with 19 clinical PeV-A1 isolates followed by staining with scFv-71 antibody and rabbit anti-FLAG antibody followed by anti-rabbit Alexa Fluor 488 secondary antibody. Nuclei were stained with DAPI. Stained cells were visualized using EVOS FL Auto imaging microscope (10 × magnification).