Figure 4

Detection of PeV types 1 to 6 using scFv-71 in IFA. HT-29 cells were mock-infected or infected with PeV types 1 to 6 and stained with scFv-71 antibody followed by staining with rabbit anti-FLAG antibody and anti-rabbit Alexa Fluor 488 secondary antibody. Nuclei were stained with DAPI. Stained cells were visualized using EVOS FL Auto imaging microscope (10 × magnification). Virus strains used in the panel: PeV-A1 “Harris”, PeV-A2 “Williamson”, PeV-A3 “152037”, PeV-A4 “K2511776”, PeV-A5 “20552322” and PeV-A6 “89”.