Figure 7 | Scientific Reports

Figure 7

From: Extracellular vesicles derived from GMSCs stimulated with TNF-α and IFN-α promote M2 macrophage polarization via enhanced CD73 and CD5L expression

Figure 7

Recombinant CD5L enhanced M2 macrophage polarization. (a) Experimental diagram for CD14+ peripheral blood-derived monocytes (PBMC)-differentiated macrophage stimulation using recombinant human CD5L protein. After monocytes differentiate macrophages, cells were treated with LPS (10 ng/mL) plus of IFN-γ (25 ng/mL) (LPS/IFN), IL-4 plus IL-13 (25 ng/mL, each; IL-4/13) or of recombinant CD5L (1 μg/mL) for 72 h, respectively. (b) Effects of CD5L stimulation on CD86 expression in macrophages. (c) Percentage of double positive cells (CD11b+ CD206+) was analyzed to measure the ratio of macrophages polarized to M2 phenotype and M2/M1 balance was determined by using the ratio of (CD11b+ CD206+)/(CD11b+ CD86+) macrophage population. *P < 0.05; **P < 0.01; ***P < 0.001. Error bars represent mean ± SD, n = 3. The significance of differences between groups was determined using one-way Tukey's test.

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