Figure 1
Method validation. (A) Illustration of the S-PIE underlying principle. Let P be the probability that a peptide with a fluorescence intensity larger than some threshold (I0) is a false positive. Then for two consecutive peptides the probability of both being false positive is equal to P^2. The same holds for longer stretches, which enable the identification of most probable epitopes. (B) Two-dimensional plot of peptides fluorescence intensity and their S-PIE analysis. (C) Heatmap showing the module-trait weighted relationships between the identified modules in Whole proteome peptide discovery array and the trait indicator of samples. The color scale on the top shows a module-trait relationship from − 1 (blue) to 1 (red), where blue represents a perfect negative correlation and red a perfect positive correlation.
