Figure 7

Characterization of FMNL1 expressing cells in renal biopsies. (A) Serial sections of PLA2R1 negative MN were immunostained with FMNL1, CD68 (monocyte/macrophage marker), CD3 (lymphocyte T marker) and CD79a (lymphocyte B marker) antibodies. Glomerular circulating cells expressing FMNL1 were CD68 positive and CD3 and CD79a negative. Original magnification 320x, scale bar: 50 µm. (B) PLA2R1 negative MN section was double immunostained with FMNL1 and CD68 Representative micrographs of glomerular circulating cells positively stained with FMNL1 (brown) and CD68 (red). Boxed area is enlarged in the right side of the figure. Magnification 320x, scale bar: 50 µm; Boxed area magnification 1600 x, scale bar:10 µm.