Figure 3 | Scientific Reports

Figure 3

From: Stimulated emission depletion microscopy with a single depletion laser using five fluorochromes and fluorescence lifetime phasor separation

Figure 3

Three color image with spectrally separated SPY555-actin (green) and antibody stainings against vimentin (Alexa Fluor 594, orange) and nuclear pores (CF680R, magenta), all depleted with 775 nm. (a) confocal, (b) STED (c) Tau-STED. This dye combination could be supplemented with e.g. Abberior STAR 635P to obtain four spectrally separable fluorochromes. Scale bar: 2 µm. The three original full images are shown in Supplemental Fig. 1.

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