Figure 6 | Scientific Reports

Figure 6

From: Stimulated emission depletion microscopy with a single depletion laser using five fluorochromes and fluorescence lifetime phasor separation

Figure 6

Fluorescence lifetime changes with distance to the coverslip. Parts of raw images of the far red channel from a five color sample at different sample depths (scale bar 2 µm) and their respective phasor plots. The color in the microscopic images codes the average lifetime of the pixels. (a) is near to the coverslip, (b–d) each 0.5 µm further into the sample. The arrows over the phasor plots always point to the same position. Near the coverslip in (a), ATTO 647N labeled phalloidin produces a separate peak to the left in phasor plot that dwindles further into the sample due to the reduced amount of actin at higher sample depths. Note the movement of the Abberior STAR 635P contribution in the phasor plot (major peak) relative to the arrow.

Back to article page