Figure 7

STED with three spectrally separated colors and corresponding crosstalk to other channels. (a) Example color overlay of the three channels. Vimentin Alexa Fluor 594 (green), mitochondria Abberior STAR 635P (red), and nuclear pores CF680R (magenta). Scale bar 2 µm. (b) Contribution chart, n = 11 image sets.