Figure 3

Caco-2 uptake of BDVs. Caco-2 cells were grown on 8-chamber glass tissue culture slides, coverslips mounted, and incubated with Band 1 (a–f), Band 2 (g–l), or Band 3 (m–r) at 5 and 22 μg/mL for 2 or 24 h. After each time point, cells were observed by confocal microscopy (c,f,i,l,o,r) or by epifluorescence (all the remaining panels). White arrows and yellow arrowheads indicate single small and large dots around nuclei respectively. A thick arrow in (f) indicates a point of entrance into an island of numerous BDVs. Bar in (a,b,d,e,g,h,j,m,n,p,q) = 10 μm; bar in (c,f,i,l,o,r) = 25 μm.