Figure 4 | Scientific Reports

Figure 4

From: Characterization of anti-proliferative and anti-oxidant effects of nano-sized vesicles from Brassica oleracea L. (Broccoli)

Figure 4

NCI-H441 uptake of BDVs. NCI-H441 cells were grown on 8-chamber glass tissue culture slides, coverslips mounted, and incubated with Band 1 (a–f), Band 2 (g–l), or Band 3 (m–r) at 5 and 22 μg/mL for 2 or 24 h. After each time point, cells were observed by confocal microscopy (c,f,i,l,o,r) or by epifluorescence (all the remaining panels). White arrows indicate single dots around nuclei. Yellow arrows point to a single cell with two or more dots around its nucleus. White arrowheads indicate diffuse perinuclear staining. Bar in (a,b,d,e,g,h,j,m,n,p,q) = 10 μm; bar in (c,f,i,l,o,r) = 25 μm.

Back to article page