Figure 5

SHSY5Y uptake of BDVs. SHSY5Y cells were grown on 8-chamber glass tissue culture slides, coverslips mounted, and incubated with Band 1 (a–f), Band 2 (g–l), or Band 3 (m-r) at 5 and 22 μg/mL for 2 or 24 h. After each time point, cells were observed by confocal microscopy (c,f,i,l,o,r) or by epifluorescence (all the remaining panels). White arrows indicate single dots around nuclei. Yellow arrowheads indicate larger dots. Yellow arrows point to a single cell with two or more dots around its nucleus. Bar in (a,b,d,e,g,h,j,m,n,p,q) = 10 μm; bar in (c,f,i,l,o,r) = 25 μm.