Figure 2
From: Non-apoptotic cell death induction via sapogenin based supramolecular particles
AG-08 is related with endosomal pathway. (A) After pre-treatment with 20 µM pitstop II, 0.5 mM methyl-β-cyclodextrin (MβC), 80 µM dynasore, 20 µM cytochalasin d (Cyto D) or 20 µM chlorpromazine (CPZ) for 1 h, HCC1937 cells were treated with 8 µM AG-08 for 24 h. Then cell viability was determined by using WST-1. Reported values were normalized on cells treated with only AG-08. Error bars are the standard deviations (n = 3). p-values were calculated with respect to AG-08 treated cells by two-tailed equal variance Student’s t-test (*p < 0.05, ***p < 0.001, ****p < 0.0001) (B) HCC1937 cells were treated with brefeldin A (Bref A) for 1 h and then treated with 8 µM AG-08 for 24 h. Error bars represent standard deviations (n = 3). p-values were calculated with respect to AG-08-treated cells (*p < 0.05, **p < 0.005, ***p < 0.001). (C) Following AG-08 treatment for 16 h, EEA1 proteins were stained using the anti-EEA1 antibody in HCC1937 cells (scale bar = 25 µm).
