Figure 3
From: Non-apoptotic cell death induction via sapogenin based supramolecular particles
mRNA microarray analysis was used to identify differentially expressed genes via AG-08 treatment. (A) Heatmap representing the expressions of 193 genes with significant different expression in AG-08 treated cells compared with control. Heatmap was generated by GraphPad Prism version 8 (https://www.graphpad.com/scientific-software/prism/). (B) Protein–protein network analysis was formed via STRING. The thickness of the connecting lines indicates the strength of data support for physical and/or functional associations. (C) Pathway enrichment was determined using GO, KEGG, and Reactome pathway analysis. The figure displays six terms with the smallest p-values. Size of circle represents the significance of enrichment. (D) The protein levels of CHOP were determined via IB. 0.5 µg/ml tunicamycin (Tun) was used as positive control. The experiments were repeated three times independently; with one representative result shown. (E) mRNA level of the CHOP was investigated using quantitative PCR (q-RTPCR). Error bars represent standard error (s.e.). p-values were calculated with respect to vehicle-treated cells by one-way Anova test. (**p < 0.005, ****p < 0.0001). (F) The expression and localization of Calnexin protein were evaluated using immunofluorescence via anti-Calnexin antibody (scale bar 25 µm).
