Table 5 Summary of expert opinions after the first round.

Methods of induction

① Increase of ionizing radiation; ② Water scald is better to master, and the consistency of animals is better, but the time of 3rd degree burns should be explored, and it may not reach 3rd degree burns; ③ the two methods of water bath scald and solid thermal scald are easier to control and standardize, and have high repeatability; ④ Adding more accurate positioning equipment such as laser thermal burn equipment should be considered

Burn sites

None

Induction temperature

None

Anesthesia

None

Skin preparation

%1Veet Hair Removal Cream Normal Skin; ② The hair of the rat model with 3rd degree burns can be shaved with an electric razor, and there is no need for chemical drugs to completely remove the hair

Housing post surgery

① Normal SPF animal house environment (barrier system), temperature: 20 ~ 25 °C, relative humidity: 40–70%, ventilation rate: 10–15 times/hour, lighting time: 12 h/12 h alternate lighting every day, feed: SPF Special, drinking water: disinfection. It is best to carry out only one experiment in each house to avoid cross-infection; ② The maintenance of wounds after injury are most relevant to temperature/humidity and stocking density, and is not closely related to the feed and drinking water

Intervention post surgery

① Try not to intervene during the experiment, so as not to cause other factors to interfere with the results and cause misjudgment. The success of the experiment is best guaranteed by environmental control

Assessment criteria

① Increase pathological section observation; ② Model construction resource consumption and model construction time consumption, the former includes the latter, and it is recommended to refine the concept of the former; ③ It is recommended to increase the parameters of scalding pressure and scalded area. Added wound healing assay and eschar removal time assay