Figure 6
From: Impact of habitual chewing on gut motility via microbiota transition
Effect of sivelestat (50 mg/kg, i.p.), a neutrophil elastase inhibitor, on the constipation-like symptoms of long-term powdered diet-fed mice. (a) Fecal pellet count, total fecal weight, and fecal moisture content (p = 0.0344, 0.0314, and 0.0140, respectively, n = 7 per group), (b) PCR analysis of Elane, IL-1β and IL-6 in the colons of mice (p = 0.0388, 0.0300, and 0.0136, respectively, n = 5 per group), and (c) western blotting analysis of Ly-6G, aquaporin-4 (AQP-4), and neural nitric oxide synthase (nNOS) in the colons of the mice (p = 0.3055, 0.1388, and 0.0399, respectively, n = 3 per group). Full length blots are presented in Supplementary Fig. S6. The data are given as the mean ± SEM for a group. Student’s t-test was used to determine statistical significance. *p < 0.05; vs. vehicle-treated powdered diet-fed mice group.
