Figure 7
From: Impact of habitual chewing on gut motility via microbiota transition

Effect of 2-h masticatory activity on the constipation-like symptoms of long-term powdered diet-fed mice. (a) Fecal pellet output (p = 0.0214, n = 13 per group) and (b) western blotting analysis of Ly-6G, aquaporin 4, and neural nitric oxide synthase (nNOS) expression (p = 0.0234, 0.0201, and 0.0954, respectively, n = 3 per group) in the colons of long-term powdered diet-fed mice subjected to the 2-h restraint-gnawing (R + G +) protocol. Full length blots are presented in Supplementary Fig. S7. The data are given as the mean ± SEM for a group. Student’s t-test was used to determine statistical significance. *p < 0.05; vs. R + G− group.