Figure 2

Western blot analysis for lysine modifying enzymes and chaperone complex components in cell lysates obtained from MC, EV, and KO clones. The protein levels were assessed by their immunoreactivities with the respective antibodies (Ab) relative to that of β-actin and were then shown as the change relative to LH2 expression levels in MC as 1.0. (a) LH1, (b) LH3, (c) GLT25D1, (d) CypB, (e) Fkbp65, (f) Hsp47, (g) Bip. Values represent mean ± S.D. (n = 3) from three independent experiments. Statistical differences were determined by the method described above (Fig. 1 legend). *p < 0.05, **p < 0.01, and ***p < 0.001 between MC and KO; ^#p < 0.05, ^##p < 0.01, and ^###p < 0.001 between EV and KO, respectively. Original blots are presented in Supplementary Fig. S3. LH lysyl hydroxylase, GLT25D1 glycosyltransferase 25 domain containing 1, CypB cyclophilin B, FKBP65 FK506-binding protein 65, Hsp47 heat shock protein 47, Bip immunoglobulin heavy-chain-binding protein, Ab antibody, MC MC3T3-E1, EV empty vector, KO knock-out.