Figure 6

In vitro mineralization assay. (A) MC, EV, and KO clones were cultured in mineralization medium for 4 weeks. The cells/matrices were stained with Alizarin Red S. In the present study, an objective with a magnifying power of 4 was used to obtain the images. Scale bar represents 100 µm. (B) Quantification of Alizarin Red S contents. The contents were measured by absorbance at 405 nm. Values represent mean ± S.D. (n = 3) from three independent experiments. Statistical differences were determined by the method described above (Fig. 1. legend). ***p < 0.001 between MC and KO; ^###p < 0.001 between EV and KO. MC MC3T3-E1, EV empty vector, KO knock-out.